|
|
| Gene : |
CD34 |
| Gene ID : |
947 |
| Uniprot ID : |
P28906 |
| Alternative Name : |
CD34 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
QBEnd/10 + HPCA1/763 |
| Isotype : |
Mouse IgG1, lambda + Mouse IgG1, lambda |
| Immunogen Information : |
Detergent solubilized vesicular suspension prepared from human term placenta (QBEnd/10); Recombinant full-length human HPCA1 protein (HPCA1/763) |
This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. Anti-CD34 labels > 85% of angiosarcoma and Kaposi s sarcoma, but with a lower specificity.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
