|
|
| Gene : |
CCND1 |
| Gene ID : |
595 |
| Uniprot ID : |
P24385 |
| Alternative Name : |
CCND1, BCL1, PRAD1 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
CCND1/809 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Recombinant human CCND1 protein |
Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Flow Cytometry (1-2ug/million cells); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
