|
|
| Gene : |
TDG |
| Gene ID : |
6996 |
| Uniprot ID : |
Q13569 |
| Alternative Name : |
Thymine-DNA Glycosylase, G/T Mismatch-Specific Thymine DNA Glycosylase, EC 3.2.2.29. |
| Format : |
Purified |
| Amount : |
20 µg |
| Clone name : |
PAT2F7A |
| Isotype : |
Mouse IgG1 heavy chain and Kappa light chain. |
| Immunogen Information : |
Anti-human TDG mAb, clone PAT2F7A, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human TDG protein 1-410 amino acids purified from E. coli. |
Thymine-DNA glycosylase (TDG) is a member of the TDG/mug DNA glycosylase family. TDG is a nuclear protein that fixes G/T mismatches to G/C pairs by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired thymin. In addition, TDG removes uracil and 5-bromouracil from mispairings with guanine. The TDG enzyme has an essential role in cellular defense against genetic mutation triggered by the spontaneous deamination of 5-methylcytosine and cytosine.
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:250.
