Figure-1: Intracellular flow cytometric analysis of TNF alpha on PMA and Ionomycin treated PBMCs using 2 µg/10^6 cells of TNF alpha (10-4114) antibody. Green represents isotype control , red represents anti-TNF alpha antibody (ABM24B7). Goat anti-mouse PE conjugate was used as secondary. The cells are lymphocyte gated.