Polyclonal Antibody to GAPDH

Product code: 11-13024

Clonality : Polyclonal
Application : WB
Reactivity : Rat, Mouse, Human

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Shipping Info:

2000 (throughout India)

Order now and get it on next 3-4 working days* (Major Cities)


Gene : GAPDH
Gene ID : 2597
Uniprot ID : P04406
Alternative Name : GAPDH, GAPD, CDABP0047, OK/SW-cl.12
Format : Purified
Amount : 100 µg
Isotype : Rabbit IgG
Immunogen Information : A partial length recombinant GAPDH protein was used as the immunogen for this antibody.

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

Purification : Protein A Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 1-2 µg/ml


Polyclonal Antibody to GAPDH

Product code: 11-13024
mg

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