Immunohistochemistry is the technique in which specific antibodies are used for in situ localization of the protein of interest in its native form. This is an extremely sensitive and specific method. The antibody-antigen interaction is visualized by employing enzymes, fluorescent probes etc. The immunohistochemistry technique has become a significant tool in diagnostics as well as in research.
Mainly there are two steps involved in this process:
a) Tissue processing
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