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|
Gene : |
ACTA2 |
Gene ID : |
59 |
Uniprot ID : |
P62736 |
Alternative Name : |
ACTA2, ACTSA, ACTVS, GIG46 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
1A4 + ACTA2/791 |
Isotype : |
Mouse IgG2a, kappa + Mouse IgG2a, kappa |
Immunogen Information : |
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjµgated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791) |
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Flow Cytometry (1-2ug/million cells); ,Immunofluorescence (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);