Monoclonal Antibody to Actin, Smooth Muscle (Leiomyosarcoma Marker)(Clone : ACTA2/791)

Product code: 36-1655

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human, Rat

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


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Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : ACTA2
Gene ID : 59
Uniprot ID : P62736
Alternative Name : ACTA2, ACTSA, ACTVS, GIG46
Format : Purified
Amount : 100 µg
Clone name : ACTA2/791
Isotype : Mouse IgG2a
Immunogen Information : Recombinant full-length human ACTA2 protein
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to Actin, Smooth Muscle (Leiomyosarcoma Marker)(Clone : ACTA2/791)

Product code: 36-1655
mg

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