|
|
| Gene : |
ACTA2 |
| Gene ID : |
59 |
| Uniprot ID : |
P62736 |
| Alternative Name : |
ACTA2, ACTSA, ACTVS, GIG46 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
ACTA2/791 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Recombinant full-length human ACTA2 protein |
Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
