|
|
| Gene : |
BAX |
| Gene ID : |
581 |
| Uniprot ID : |
Q07812 |
| Alternative Name : |
BAX, BCL2L4 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM336 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. |
Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); ,Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes),
