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|
Gene : |
BCL2L1 |
Gene ID : |
598 |
Uniprot ID : |
Q07817 |
Alternative Name : |
BCL2L1, BCL2L, BCLX |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
2H12 |
Isotype : |
Mouse IgG2a, kappa |
Immunogen Information : |
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein |
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)