|
|
| Gene : |
PTPRC |
| Gene ID : |
5788 |
| Uniprot ID : |
P08575 |
| Alternative Name : |
PTPRC, CD45 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
UCHL-1 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Cultured human T-cells from an IL-2-dependent T-cell line (CA1) |
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
