Monoclonal Antibody to CD54 / ICAM-1(Clone : W-CAM-1; same as Wehi-CAM-1 or 1H4)

Product code: 36-1251

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human

Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


Bulk Order


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : ICAM1
Gene ID : 3383
Uniprot ID : P05362
Alternative Name : ICAM1
Format : Purified
Amount : 100 µg
Clone name : W-CAM-1; same as Wehi-CAM-1 or 1H4
Isotype : Mouse IgG2b Kappa
Immunogen Information : Raji Burkitt lymphoma cells
Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This MAb blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Functional Studies (Order Ab without BSA & Azide); Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (2-4µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to CD54 / ICAM-1(Clone : W-CAM-1; same as Wehi-CAM-1 or 1H4)

Product code: 36-1251
mg

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