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|
Gene : |
CD63 |
Gene ID : |
967 |
Uniprot ID : |
P08962 |
Alternative Name : |
CD63, MLA1, TSPAN30 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
NKI/C3 + LAMP3/968 |
Isotype : |
Mouse IgG1, kappa + Mouse IgG1, kappa |
Immunogen Information : |
Smooth plasma membrane fraction of MeWo cells (NKI/C3); Recombinant human full-length CD63 protein (LAMP3/968) |
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);