|
|
| Gene : |
CD74 |
| Gene ID : |
972 |
| Uniprot ID : |
P04233 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
CLIP/813 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Recombinant full-length human CD74 protein |
It recognizes a protein of ~35kDa, identified as CD74. It is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Flow Cytometry (1-2ug/million cells) (Methanol Fixed); ,Immunofluorescence (1-2ug/ml); Western Blot (2-4ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
