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|
| Gene : |
KRT8 |
| Gene ID : |
3856 |
| Uniprot ID : |
P05787 |
| Alternative Name : |
KRT8, CYK8 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
B22.1/B23.1 |
| Isotype : |
Mouse IgG1, kappa + Mouse IgG1, kappa |
| Immunogen Information : |
Cytoskeletal prepaRation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1) |
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.?Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
