Monoclonal Antibody to Cytokeratin 8/18(KRT8/803 + KRT18/835)

Product code: 36-11031

Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


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Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : KRT8
Gene ID : 3856
Uniprot ID : P05787
Alternative Name : KRT8, CYK8
Format : Purified
Amount : 100 µg
Clone name : KRT8/803 + KRT18/835
Isotype : Mouse IgG
Immunogen Information : Recombinant full-length human KRT8 protein (KRT8/803); Recombinant full-length human KRT18 protein (KRT18/835)
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.?Immuno-histochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to Cytokeratin 8/18(KRT8/803 + KRT18/835)

Product code: 36-11031
mg

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