Monoclonal Antibody to Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(Clone : KRTL/1077)

Product code: 36-11115

Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human, Rat. Shows broad species reactivity

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : KRT77
Gene ID : 374454
Uniprot ID : Q7Z794
Alternative Name : KRT77, KRT1B
Format : Purified
Amount : 100 µg
Clone name : KRTL/1077
Isotype : Mouse IgG1 Kappa
Immunogen Information : Recombinant human KRT77 protein
This MAb recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40kDa. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Western Blot (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 min at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(Clone : KRTL/1077)

Product code: 36-11115
mg

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