Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Product code: 10-10011

Clonality : Monoclonal
Application : IHC, WB
Reactivity : Mouse, Human

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Gene : GAPDH
Gene ID : 2597
Uniprot ID : P04406
Alternative Name : GAPDH, GAPD, CDABP0047, OK/SW-cl.12
Format : Purified
Amount : 100 µg
Clone name : ABM22C5
Isotype : Mouse IgG1 Kappa
Immunogen Information : A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml

1. Kakade PS, Budnar S, Kalraiya RD, Vaidya MM. Functional Implications of O-GlcNAcylation-dependent Phosphorylation at a Proximal Site on Keratin 18. J Biol Chem. 2016 Jun 3;291(23):12003-13. doi: 10.1074/jbc.M116.728717. Epub 2016 Apr 8. PubMed PMID: 27059955.

2. Rohit Shetty, Anupam Sharma, Natasha Pahuja, Priyanka Chevour, Neeraja Padmajan, Kamesh Dhamodaran, Chaitra Jayadev, Rudy M. M. A. Nuijts, Arkasubhra Ghosh, Jeyabalan Nallathambi. Oxidative stress induces dysregulated autophagy in corneal epithelium of keratoconus patients. PLoS One. 2017; 12(9): e0184628. Published online 2017 Sep 13. doi: 10.1371/journal.pone.0184628 PMCID: PMC5597215

3. Ashraf R, Hamidullah, Hasanain M, Pandey P, Maheshwari M, Singh LR, Siddiqui MQ, Konwar R, Sashidhara KV, Sarkar J. Coumarin-chalcone hybrid instigates DNA damage by minor groove binding and stabilizes p53 through post translational modifications. Sci Rep. 2017 Mar 28;7:45287. doi: 10.1038/srep45287. PubMed PMID: 28349922; PubMed Central PMCID: PMC5368660.

4. Kumar S, Singh U, Goswami C, Singru PS. Transient receptor potential vanilloid 5 (TRPV5), a highly Ca(2+) -selective TRP channel in the rat brain: relevance to neuroendocrine regulation. J Neuroendocrinol. 2017 Apr;29(4). doi: 10.1111/jne.12466. PubMed PMID: 28235149.

5. Sahay P, Rao A, Padhy D, Sarangi S, Das G, Reddy MM, Modak R. Functional Activity of Matrix Metalloproteinases 2 and 9 in Tears of Patients With Glaucoma. Invest Ophthalmol Vis Sci. 2017 May 1;58(6):BIO106-BIO113. doi: 10.1167/iovs.17-21723. PubMed PMID: 28586796.

6. Nayak TK, Mamidi P, Kumar A, Singh LP, Sahoo SS, Chattopadhyay S, Chattopadhyay S. Regulation of Viral Replication, Apoptosis and Pro-Inflammatory Responses by 17-AAG during Chikungunya Virus Infection in Macrophages. Viruses. 2017 Jan 6;9(1). pii: E3. doi: 10.3390/v9010003. PubMed PMID: 28067803; PubMed Central PMCID: PMC5294972.

7. Panigrahi B, Singh RK, Mishra S, Mandal D. Cyclic peptide-based nanostructures as efficient siRNA carriers. Artif Cells Nanomed Biotechnol. 2018 Oct 12:1-11. doi: 10.1080/21691401.2018.1511574. [Epub ahead of print] PubMed PMID: 30311806.

8. Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux. S Shivakumar, T Panigrahi, R Shetty, M Subramani, A Ghosh, N Jeyabalan. BioMed Research International, 2018.

9. Pandey P, Singh D, Hasanain M, Ashraf R, Maheshwari M, Choyal K, Singh A, Datta D, Kumar B, Sarkar J. 7-hydroxyfrullanolide, isolated from Sphaeranthus indicus, inhibits colorectal cancer cell growth by p53 dependent and independent mechanism. Carcinogenesis. 2018 Dec 8. doi: 10.1093/carcin/bgy176. [Epub ahead of print] PubMed PMID: 30535334.

Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Product code: 10-10011

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