|
|
| Gene : |
MLANA |
| Gene ID : |
2315 |
| Uniprot ID : |
Q16655 |
| Alternative Name : |
MLANA, MART1 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
A103 + HMB45 + T311 |
| Isotype : |
Mouse IgG1, kappa + Mouse IgG2a, kappa + Mouse IgG1, kappa |
| Immunogen Information : |
Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45) |
This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Western Blot (2-4ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
