Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : SPM132)

Product code: 36-1448

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : MUC1
Gene ID : 4582
Uniprot ID : P15941
Alternative Name : MUC1, PUM
Format : Purified
Amount : 100 µg
Clone name : SPM132
Isotype : Mouse IgG1, kappa
Immunogen Information : Human milk fat globule membranes
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The β subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);


Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : SPM132)

Product code: 36-1448
mg

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