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|
| Gene : |
NEFH |
| Gene ID : |
4744 |
| Uniprot ID : |
P12036 |
| Alternative Name : |
NEFH, KIAA0845, NFH |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
RT-97 + NR-4 |
| Isotype : |
Mouse IgG1, kappa + Mouse IgG1, kappa |
| Immunogen Information : |
Triton-X 100 insoluble proteins of Rat brain (RT-97); Neurofilaments from porcine spinal cord (NR-4) |
This MAb reacts with a 200kDa and 68kDa protein, identified as heavy and light sub-units of neurofilaments (NF-H & NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Flow Cytometry (1-2ug/million cells); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
