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Gene : |
CDKN1B |
Gene ID : |
1027 |
Uniprot ID : |
P46527 |
Alternative Name : |
CDKN1B, KIP1 |
Format : |
Purified |
Amount : |
100 µg |
Clone name : |
KIP1/769 |
Isotype : |
Mouse IgG1, kappa |
Immunogen Information : |
Recombinant human CDKN1B protein |
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);