|
|
| Gene : |
PCNA |
| Gene ID : |
5111 |
| Uniprot ID : |
P12004 |
| Alternative Name : |
PCNA |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM350 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Rat PCNA/Protein A fusion protein |
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
