Monoclonal Antibody to Vimentin (Mesenchymal Cell Marker)(Clone : VM1170)

Product code: 36-1802

Clonality : Monoclonal
Application : FACS, IF, WB, IHC
Reactivity : Human

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : VIM
Gene ID : 7431
Uniprot ID : P08670
Alternative Name : VIM
Format : Purified
Amount : 100 µg
Clone name : VM1170
Isotype : Mouse IgG1
Immunogen Information : Recombinant full-length human vimentin protein
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.?Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Does not react with mouse and Rat.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Western Blot (0.25-0.5µg/ml); Immunohistology (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to Vimentin (Mesenchymal Cell Marker)(Clone : VM1170)

Product code: 36-1802
mg

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