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| Gene : |
ZAP70 |
| Gene ID : |
7535 |
| Uniprot ID : |
P43403 |
| Alternative Name : |
ZAP70, SRK |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
2F3.2 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 |
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. ?Control of this protein translation is via the IgVH gene.?In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. ?Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Flow Cytometry (1-2ug/million cells);Immunofluorescence (1-2ug/ml);Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
