Monoclonal Antibody to ZAP70 (Chronic Lymphocytic Leukemia Marker)(Clone : 2F3.2)

Product code: 36-1812

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human

Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


Bulk Order


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : ZAP70
Gene ID : 7535
Uniprot ID : P43403
Alternative Name : ZAP70, SRK
Format : Purified
Amount : 100 µg
Clone name : 2F3.2
Isotype : Mouse IgG2a
Immunogen Information : Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. ?Control of this protein translation is via the IgVH gene.?In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. ?Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to ZAP70 (Chronic Lymphocytic Leukemia Marker)(Clone : 2F3.2)

Product code: 36-1812
mg

Can't read the image? click here to refresh.