Core diameter: 2.0 mm
Section thickness: 4 micrometer
Slide orientation:
In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. #60 location is usually filled with carbon for orientation.
How processed
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Tissues were initially fixed with formalin except for some of the animal tissues.
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Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%, 95%, 99%, 100% x 3 times.
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Cleared by xylene, three changes for 1 hour each.
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Infiltrated with 60oC paraffin, three changes for 1 hour each
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Sectioned by microtome in 4 micro meter thickness and put on Superfrost plus slides.
Before use
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Dry slides for 1 hour in a oven at 60 degree.
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Dewax slides in xylene for 4 minutes x 5 times.
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Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
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Immerse slides in tap water for 5 minutes.
Applications
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Immunohistochemistry
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Fluorescent in situ hybridization (FISH)
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mRNA in situ hybridization
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miRNA in situ hybridization
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TUNEL for apoptosis
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Nucleic acid extraction
