|
|
Alternative Name : |
NT4, NT5, NTF5, NT-4/5, NTF4, Neurotrophin-4, Neutrophic factor 4, Neurotrophin-5, NT-5. |
Amount : |
10 µg |
Source : Escherichia Coli.
Neurotrophin-4 Human Recombinant produced in E.Coli is a noncovalently linked homodimer, non-glycosylated polypeptide chain containing 2 x 130 amino acids (81-210 amino acids) and having a total molecular mass of 28 kDa. The NT-4 is purified by proprietary chromatographic techniques.
NT-4 is part of the family of neurotrophic factors, neurotrophins, that are in charge for the survival and differentiation of mammalian neurons. NT-4 expression is dominant and less influenced by environmental signals. NT-4 deficient mice shows slight cellular deficits and develop normally to adulthood. NT-4 is a target-derived survival factor for peripheral sensory sympathetic neurons.NT-4 is involved in the proliferation and differentiation of periodontal ligament cells.
It is recommended to reconstitute the lyophilized NT-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found to be in the range of 20-50 ng/ml.