|
|
Gene : |
APAF1 |
Gene ID : |
317 |
Uniprot ID : |
O14727 |
Alternative Name : |
APAF1, KIAA0413 |
Format : |
Sera |
Amount : |
50 µl |
Isotype : |
Rabbit IgG |
Immunogen Information : |
A synthetic peptide of APAF-1 protein (amino acids 264-282 GPKYVVPVESSLGKEKGLE) was used as the immunogen for this antibody |
Apaf 1 (apoptosis protease-activating factor-1) is a key regulator of the mitochondrial apoptotic pathway, being the central element of the multimeric apoptosome.� The apoptosome consists of cytochrome c, procaspase-9 and seven Apaf 1 monomers, and is considered to�be core apoptotic machinery�that executes mitochondria-dependent apoptosis. Cytochrome c, normally compartmentalized in the mitochorndria, is released into the cytoplasm following apoptotic stimuli.�� Apaf 1 binds cytochrome c in the cytoplasm and in the presence of dATP/ATP forms the apoptosome.� The apoptosome binds procaspase-9 and promotes its autocatalytic activation.� Active caspase-9, in turn, activates downstream caspases including 3, 6, and 7 contributing to the proteolytic caspase activation cascade which leads to cell death. �
WB: 1:1000-1:2000, IHC (paraffin): 1:1000-1:5000, IHC (frozen): Users should optimize, IP: 1:50-1:200