Custom Antibody Development/Production/Generation Services In India

ABGENEX provides a full range of custom antibody development/production services in India for the life science and biotech industry. We have well-equipped laboratories for the development of Polyclonal and Monoclonal antibody in India. We take extra care for custom projects initiated with ABGENEX. Our expert team having more than 12 years of expertise in custom antibody development/production, carefully evaluates and continuously tracks all projects aiming for successful completion in a time-bound manner.

Custom Polyclonal Antibody Development/Production Service in Rabbit (2 New Zealand white rabbits)

ABGENEX a leading custom polyclonal antibody developing company in India, develops polyclonal antibodies from synthetic peptide, recombinant protein, gel-eluted protein, gel embedded protein (gel piece), purified protein etc. We also synthesize synthetic peptides and recombinant protein for the development of polyclonal antibody in India. 

Before initiating a custom polyclonal antibody development/production project  with us, customer needs to download the “Immunogen Form” by clicking here  and send us back the filled immunogen form along with the antigen. Confidentiality about the protein (if known) is guaranteed.

Salient Features of Custom Polyclonal Antibody Development/Production Service

  • 12 Weeks protocol to produce antibody in 2 New Zealand White Rabbits (From confirmed Pure line Parental stock).
  • This will include 7 immunizations and 3 Production bleeds per animal.
  • Pre-immune sera of 5-10 ml per animal and a total of 30-40 ml of crude immune sera per rabbit.
  • *FREE ELISA Titer at first bleed against free immunogen.
Customer will be notified of immunization schedule and tentative date of production/Progress of the Project etc.

Immunogen required for custom polyclonal antibody development/production 
  1. For Protein (Provided by customer)-3 mg, Concentration: minimum 0.5 mg/ml
  2. For peptide (Min ~5-7 mg)
Week 1 (Pre-bleed)
Primary Immunization
Pre-Immune Sera and Primary Immunization ~5 ml*
Week 2 (Immunize) 2nd Immunization  
Week 3 (Immunize) 3rd Immunization  
Week 4 (Immunize) 4th Immunization  
Week 5 (Immunize) 5th Immunization  
Week 6 (Bleed-1) 1st production Bleed ~10 ml*
Week 7 (Immunize) 6th Immunization  
Week 8 (Bleed-2) 2nd production Bleed ~10 ml*
Week 9 (Immunize) 7th Immunization  
Week 10 (Bleed-3) 3rd production Bleed ~10 ml*
Extention of project per month (2 bleeds/Rabbit) Bleed-1 ~10 ml*
Bleed-2 ~10 ml*
Terminal bleed
(1 bleed per Rabbit)
Bleed-1 ~20-25 ml*


* In our custom polyclonal development/production service we usually provide the above mentioned sera from one rabbit/project but it may vary depending upon the health status of the animal.

The above schedule of custom polyclonal antibody development/production is approximate. We can also modify it according to the customer’s need.

Additional 4 weeks is required if the custom polyclonal antibody development/production service requires peptide synthesis, mass spec, KLH conjugation. For custom polyclonal antibody development service in which the immunogen is supplied can be started immediately. 

Protein A : 15-20 mg of purified Antibody
Peptide affinity : 2-3 mg of purified Antibody
Additional Services

  • Custom peptide synthesis
  • Recombinant protein production for custom polyclonal antibody development/production in India
  • Custom polyclonal antibody development service against Phospho/Acetylated protein in India
  • Custom polyclonal antibody development service in Mice/Rat in India
  • Validation of custom polyclonal antibody for FACS, IP, IHC and IF
  • Custom monoclonal antibody development service in India
Terms and Conditions:
  1. If the immunogen is peptide then it must contain a cysteine for conjugation.
  2. If the immunogen is already conjugated or it’s in gel, kindly provide us ~1 mg of free peptide/purified protein for ELISA.
  3. Recombinant protein and purified protein must be shipped in dry ice.
  4. Kindly provide antigen in soluble form.
  5. For peptide affinity purification customer should provide additional 6 mg peptide.
  6. ABGENEX guarantees ELISA positive sera for the immunogen synthesized/supplied by ABGENEX.
  7. The titre of the antisera depends on the quality of the antigen.

List of publications of custom polyclonal antibody development service in India

  1. Kshirsagar R, Ghodke I, Muniyappa K. Saccharomyces cerevisiae Red1 protein exhibits nonhomologous DNA end joining activity and potentiates Hop1 promoted pairing of double-stranded DNA. Journal of Biological Chemistry. 2017 Jun 22:jbc-M117. PubMed PMID:28642366 ( Application: Western blot

  2. Kumar V, Yadav S, Soumya N, Kumar R, Babu NK, Singh S. Biochemical and inhibition studies of glutamine synthetase from Leishmania donovani. Microbial Pathogenesis. 2017 Jun 30;107:164-74. PubMed PMID:28351708. ( Application: Western blot & Immunolocalization

  3. Bhardwaj R, Das M, Singh S, Chiranjivi AK, Prabhu SV, Singh SK, Dubey VK. Evaluation of CAAX prenyl protease II of Leishmania donovani as potential drug target: Infectivity and growth of the parasite is significantly lowered after the gene knockout. European Journal of Pharmaceutical Sciences. 2017 May 1;102:156-60. PubMed PMID: 28279761. ( Application: Western blot & Immunofluorescence

  4. Ghodke I, Muniyappa K. Genetic and biochemical evidences reveal novel insights into the mechanism underlying Saccharomyces cerevisiae Sae2-mediated abrogation of DNA replication stress. Journal of biosciences. 2016 Dec 1;41(4):615-41. PubMed PMID:27966484 ( Application: Western blot & FACS

  5. Chakraborty C, Roychowdhury A, Samadder S, Roy A, Mandal RK, Basu P, Roychoudhury S, Panda CK. Association of P16-RBSP3 inactivation with phosphorylated RB1 overexpression in basal–parabasal layers of normal cervix unchanged during CACX development. Biochemical Journal. 2016 Oct PubMed PMID:27458253 ( Application: IHC & Immunofluorescence

  6. Dey A, Samanta MK, Gayen S, Maiti MK. The sucrose non-fermenting 1-related kinase 2 gene SAPK9 improves drought tolerance and grain yield in rice by modulating cellular osmotic potential, stomatal closure and stress-responsive gene expression. BMC plant biology. 2016 Jul 13;16(1):158. Application: Western blot

  7. Petla BP, Kamble NU, Kumar M, Verma P, Ghosh S, Singh A, Rao V, Salvi P, Kaur H, Saxena SC, Majee M. Rice PROTEIN l‐ISOASPARTYL METHYLTRANSFERASE isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity. New Phytologist. 2016 Jul 1;211(2):627-45. PubMed PMID 26987457 ( Application: Western blot & Immunolocalization

  8. Manjegowda MC, Gupta PS, Limaye AM. Validation data of a rabbit antiserum and affinity purified polyclonal antibody against the N-terminus of human GPR30. Data in brief. 2016 Jun 30;7:1015-20. PubMed PMID:27408914 ( Application: Indirect ELISA  & Western blot

  9. Bhardwaj R, Kumar R, Singh SK, Selvaraj C, Dubey VK. Understanding the importance of conservative hypothetical protein LdBPK_070020 in Leishmania donovani and its role in subsistence of the parasite. Archives of biochemistry and biophysics. 2016 Apr 15;596:10-21. PubMed PMID:26926257 ( Application: Western blot, FACS

  10. Soumya N, Tandan H, Damre MV, Gangwal RP, Sangamwar AT, Singh S. Leucine-684: A conserved residue of an AMP-acetyl CoA synthetase (AceCS) from Leishmania donovani is involved in substrate recognition, catalysis and acetylation. Gene. 2016 Apr 15;580(2):125-33. Application: Western blot

  11. Kumar S, Mamidi P, Kumar A, Basantray I, Bramha U, Dixit A, Maiti PK, Singh S, Suryawanshi AR, Chattopadhyay S, Chattopadhyay S. Development of novel antibodies against non-structural proteins nsP1, nsP3 and nsP4 of chikungunya virus: potential use in basic research. Archives of virology. 2015 Nov 1;160(11):2749-61. PMID:26280524 ( Application: ELISA & Western blot

  12. Majumdar G, Yadav G, Rani S, Kumar V. Bird eyes distinguish summer from winter: Retinal response to acute photoperiod change in the night-migratory redheaded bunting. Journal of chemical neuroanatomy. 2015 Oct 31;68:55-60. PubMed PMID 26219493 ( Application: Immunofluorescence & IHC

  13. Sahoo SK, Chainy GB, Dandapat J. Neonatal Hypothyroidism alters Expression of Antioxidant Enzymes and Redox status in adult Rat Seminiferous Tubule Cells. Current Trends in Biotechnology and Pharmacy. 2015 Apr 1;9(2):117-31. Application: Western blot & IHC

  14. Paul A, Samaddar S, Bhattacharya A, Banerjee A, Das A, Chakrabarti S, DasGupta M. Gatekeeper tyrosine phosphorylation is autoinhibitory for Symbiosis Receptor Kinase. FEBS letters, 2014 Aug 25:588(17):2881-9. PubMed PMID:24996184 ( Application: Immuno precipitation & Western blot

  15. Srivastava G, Kayastha AM. β-Amylase from starchless seeds of Trigonella foenum-graecum and its localization in germinating seeds. PloS one. 2014 Feb 14;9(2):e88697. Application: Western blot & IHC

  16. Samaddar S, Dutta A, Sinharoy S, Paul A, Bhattacharya A, Saha S, Chien KY, Goshe MB, DasGupta M. Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase. FEBS letters. 2013 Sep 17;587(18):2972-9. PubMed PMID: 23962520. ( Application: Western blot

  17. Somyajit K, Basavaraju S, Scully R, Nagaraju G. ATM-and ATR-mediated phosphorylation of XRCC3 regulates DNA double-strand break-induced checkpoint activation and repair. Molecular and cellular biology. 2013 May 1;33(9):1830-44. PubMed Central PMCID: PMC3624173. ( Application: Western blot & Immunofluorescence

  18. Verma P, Kaur H, Petla BP, Rao V, Saxena SC, Majee M. PROTEIN L-ISOASPARTYL METHYLTRANSFERASE2 is differentially expressed in chickpea and enhances seed vigor and longevity by reducing abnormal isoaspartyl accumulation predominantly in seed nuclear proteins. Plant physiology. 2013 Mar 1;161(3):1141-57. PubMed PMID: 23284083. ( Application: Western blot 

  19. Ghodke I, Muniyappa K. Processing of DNA double-stranded breaks and intermediates of recombination and repair by Saccharomyces cerevisiae Mre11 and its stimulation by Rad50, Xrs2, and Sae2 proteins. Journal of Biological Chemistry. 2013 Apr 19;288(16):11273-86. PubMed PMID:23443654; PubMed Central PMCID: PMC3630844.( Application: Western blot

  20. Sareddy GR, Divya K, Kirti PB, Prakash Babu P. Novel antiproliferative and antioxidant role of BJANN1, a mustard annexin protein in human glioblastoma cell lines. J Cancer Sci Ther. 2013;5:256-63. ( Application: Western blot & IHC

  21. Maiti GP, Ghosh A, Chatterjee R, Roy A, Sharp TV, Roychoudhury S, Panda CK. Reduced expression of LIMD1 in ulcerative oral epithelium associated with tobacco and areca nut. Asian Pacific Journal of Cancer Prevention. 2012;13(9):4341-6. PubMed PMID: 23167340 ( Application: ELISA & IHC

  22. Ghose J, Sinha M, Das E, Jana NR, Bhattacharyya NP. Regulation of miR-146a by RelA/NFkB and p53 in STHdhQ111/HdhQ111 Cells, a Cell Model of Huntington's Disease. PLoS One. 2011 Aug 26;6(8):e23837. PubMed PMID: 21887328; PubMed Central PMCID: PMC3162608. ( Western blot

  23. Ghosh AS, Ray D, Dutta S, Raha S. EhMAPK, the mitogen-activated protein kinase from Entamoeba histolytica is associated with cell survival. PloS one. 2010 Oct 8;5(10):e13291. PubMed PMID: 20949043; PubMed Central PMCID: PMC2951911. ( Application: Western blot

  24. Bandhu A, Ganguly T, Jana B, Mondal R, Sau S. Regions and residues of an asymmetric operator DNA interacting with the monomeric repressor of temperate mycobacteriophage L1. Biochemistry. 2010 Apr 21;49(19):4235-43. PubMed PMID: 20377203. ( Application: Western blot

  25. Bhanja S, Chainy GB. PTU-induced hypothyroidism modulates antioxidant defence status in the developing cerebellum. International Journal of Developmental Neuroscience. 2010 May 31;28(3):251-62. PubMed PMID: 20123122. ( Application: Western blot

  26. Sureka K, Hossain T, Mukherjee P, Chatterjee P, Datta P, Kundu M, Basu J. Novel role of phosphorylation-dependent interaction between FtsZ and FipA in mycobacterial cell division. PLOS one. 2010 Jan 6;5(1):e8590. PubMed PMID: 20066037; PubMed Central PMCID: PMC2797604. ( Application: Western blot

  27. Sinha D, Joshi N, Chittoor B, Samji P, D'silva P. Role of Magmas in protein transport and human mitochondria biogenesis. Human molecular genetics. 2010 Jan 6;19(7):1248-62. PubMed PMID: 20053669; PubMed Central PMCID: PMC2838536. ( Application: Western blot

  28. Dey S, Banerjee P, Saha P. Cell cycle specific expression and nucleolar localization of human J-domain containing co-chaperon Mrj. Molecular and cellular biochemistry. 2009 Feb 1;322(1-2):137-42. PubMed PMID: 19002655. ( Application: Western blot & Immunofluorescence

  29. Dutta S, Sardar A, Ray D, Raha S. Molecular and functional characterization of EhPAK3, a p21 activated kinase from Entamoeba histolytica. Gene. 2007 Nov 1;402(1):57-67. PubMed PMID: 17761392. ( Application: Fluorescence spectroscopy & Western blot