Custom Antibody Development/Production/Generation Services In India

ABGENEX provides a full range of custom antibody development/production services in India for the life science and biotech industry. We have well-equipped laboratories for the development of Polyclonal and Monoclonal antibody in India. We take extra care for custom projects initiated with ABGENEX. Our expert team having more than 12 years of expertise in custom antibody development/production, carefully evaluates and continuously tracks all projects aiming for successful completion in a time-bound manner.

Custom Polyclonal Antibody Development/Production Service in Rabbit (2 New Zealand white rabbits)

ABGENEX a leading custom polyclonal antibody developing company in India, develops polyclonal antibodies from synthetic peptide, recombinant protein, gel-eluted protein, gel embedded protein (gel piece), purified protein etc. We also synthesize synthetic peptides and recombinant protein for the development of polyclonal antibody in India. 

Before initiating a custom polyclonal antibody development/production project  with us, customer needs to download the “Immunogen Form” by clicking here  and send us back the filled immunogen form along with the antigen. Confidentiality about the protein (if known) is guaranteed.

Salient Features of Custom Polyclonal Antibody Development/Production Service

  • 12 Weeks protocol to produce antibody in 2 New Zealand White Rabbits (From confirmed Pure line Parental stock).
  • This will include 7 immunizations and 3 Production bleeds per animal.
  • Pre-immune sera of 5-10 ml per animal and a total of 30-40 ml of crude immune sera per rabbit.
  • *FREE ELISA Titer at first bleed against free immunogen.
Customer will be notified of immunization schedule and tentative date of production/Progress of the Project etc.

Immunogen required for custom polyclonal antibody development/production 
  1. For Protein (Provided by customer)-3 mg, Concentration: minimum 0.5 mg/ml
  2. For peptide (Min ~5-7 mg)
IMMUNIZATION AND BLEEDING SCHEDULE
WEEKS PROCEDURE BLEED/RABBIT
Week 1 (Pre-bleed)
Primary Immunization
Pre-Immune Sera and Primary Immunization ~5 ml*
Week 2 (Immunize) 2nd Immunization  
Week 3 (Immunize) 3rd Immunization  
Week 4 (Immunize) 4th Immunization  
Week 5 (Immunize) 5th Immunization  
Week 6 (Bleed-1) 1st production Bleed ~10 ml*
Week 7 (Immunize) 6th Immunization  
Week 8 (Bleed-2) 2nd production Bleed ~10 ml*
Week 9 (Immunize) 7th Immunization  
Week 10 (Bleed-3) 3rd production Bleed ~10 ml*
 
Extention of project per month (2 bleeds/Rabbit) Bleed-1 ~10 ml*
Bleed-2 ~10 ml*
 
Terminal bleed
(1 bleed per Rabbit)
Bleed-1 ~20-25 ml*

 

* In our custom polyclonal development/production service we usually provide the above mentioned sera from one rabbit/project but it may vary depending upon the health status of the animal.

The above schedule of custom polyclonal antibody development/production is approximate. We can also modify it according to the customer’s need.

Additional 4 weeks is required if the custom polyclonal antibody development/production service requires peptide synthesis, mass spec, KLH conjugation. For custom polyclonal antibody development service in which the immunogen is supplied can be started immediately. 

Purification:
Protein A : 15-20 mg of purified Antibody
Peptide affinity : 2-3 mg of purified Antibody
Additional Services

  • Custom peptide synthesis
  • Recombinant protein production for custom polyclonal antibody development/production in India
  • Custom polyclonal antibody development service against Phospho/Acetylated protein in India
  • Custom polyclonal antibody development service in Mice/Rat in India
  • Validation of custom polyclonal antibody for FACS, IP, IHC and IF
  • Custom monoclonal antibody development service in India
Terms and Conditions:
  1. If the immunogen is peptide then it must contain a cysteine for conjugation.
  2. If the immunogen is already conjugated or it’s in gel, kindly provide us ~1 mg of free peptide/purified protein for ELISA.
  3. Recombinant protein and purified protein must be shipped in dry ice.
  4. Kindly provide antigen in soluble form.
  5. For peptide affinity purification customer should provide additional 6 mg peptide.
  6. ABGENEX guarantees ELISA positive sera for the immunogen synthesized/supplied by ABGENEX.
  7. The titre of the antisera depends on the quality of the antigen.

List of publications of custom polyclonal antibody development service in India

  1. Dutta S, Sardar A, Ray D, Raha S. Molecular and functional characterization of EhPAK3, a p21 activated kinase from Entamoeba histolytica. Gene. 2007 Nov 1; 402(1-2):57-67. Epub 2007 Aug 2. PubMed PMID: 17761392. (http://www.ncbi.nlm.nih.gov/pubmed/17761392)

  2. Dey S, Banerjee P, Saha P. Cell cycle specific expression and nucleolar localization of human J-domain containing co-chaperone Mrj. Mol Cell Biochem. 2009 Feb; 322(1-2):137-42. doi: 10.1007/s11010-008-9950-y. Epub 2008 Nov 12.PubMed PMID: 19002655. (http://www.ncbi.nlm.nih.gov/pubmed/19002655)

  3. Sinha D, Joshi N, Chittoor B, Samji P, D'Silva P. Role of Magmas in protein transport and human mitochondria biogenesis. Hum Mol Genet. 2010 Apr 1;19(7):1248-62. doi: 10.1093/hmg/ddq002. Epub 2010 Jan 6. PubMed PMID: 20053669; PubMed Central PMCID: PMC2838536. (http://www.ncbi.nlm.nih.gov/pubmed/20053669)

  4. Sureka K, Hossain T, Mukherjee P, Chatterjee P, Datta P, Kundu M, Basu J. Novel role of phosphorylation-dependent interaction between FtsZ and FipA in mycobacterial cell division. PLoS One. 2010 Jan 6;5(1):e8590. doi: 10.1371/journal.pone.0008590. PubMed PMID: 20066037; PubMed Central PMCID: PMC2797604. (http://www.ncbi.nlm.nih.gov/pubmed/?term=PMC2797604)

  5. Bandhu A, Ganguly T, Jana B, Mondal R, Sau S. Regions and residues of an asymmetric operator DNA interacting with the monomeric repressor of temperate mycobacteriophage L1. Biochemistry. 2010 May 18;49(19):4235-43. doi: 10.1021/bi9020956. PubMed PMID: 20377203. (http://www.ncbi.nlm.nih.gov/pubmed/20377203)

  6. Bhanja S, Chainy GB. PTU-induced hypothyroidism modulates antioxidant defence status in the developing cerebellum. Int J Dev Neurosci. 2010 May;28(3):251-62. doi: 10.1016/j.ijdevneu.2010.01.005. Epub 2010 Feb 1. PubMed PMID: 20123122. (http://www.ncbi.nlm.nih.gov/pubmed/20123122)

  7. Ghosh AS, Ray D, Dutta S, Raha S. EhMAPK, the mitogen-activated protein kinase from Entamoeba histolytica is associated with cell survival. PLoS One. 2010 Oct 8;5(10):e13291. doi: 10.1371/journal.pone.0013291. PubMed PMID: 20949043; PubMed Central PMCID: PMC2951911. (http://www.ncbi.nlm.nih.gov/pubmed/20949043)

  8. Ghose J, Sinha M, Das E, Jana NR, Bhattacharyya NP. Regulation of miR-146a by RelA/NFkB and p53 in STHdh(Q111)/Hdh(Q111) cells, a cell model of Huntington'sdisease. PLoS One. 2011;6(8):e23837. doi: 10.1371/journal.pone.0023837. Epub 2011 Aug 26. PubMed PMID: 21887328; PubMed Central PMCID: PMC3162608. (http://www.ncbi.nlm.nih.gov/pubmed/?term=PMID%3A+21887328)

  9. Maiti GP, Ghosh A, Chatterjee R, Roy A, Sharp TV, Roychoudhury S, Panda CK. Reduced expression of LIMD1 in ulcerative oral epithelium associated with tobacco and areca nut. Asian Pac J Cancer Prev. 2012;13(9):4341-6. PubMed PMID: 23167340 (http://www.ncbi.nlm.nih.gov/pubmed/23167340)

  10. Verma P, Kaur H, Petla BP, Rao V, Saxena SC, Majee M. PROTEIN L-ISOASPARTYL METHYLTRANSFERASE2 is differentially expressed in chickpea and enhances seed vigor and longevity by reducing abnormal isoaspartyl accumulation predominantly in seed nuclear proteins. Plant Physiol. 2013 Mar;161(3):1141-57. doi:10.1104/pp.112.206243. Epub 2013 Jan 2. PubMed PMID: 23284083. (http://www.ncbi.nlm.nih.gov/pubmed/23284083)

  11. Somyajit K, Basavaraju S, Scully R, Nagaraju G. ATM- and ATR-mediated phosphorylation of XRCC3 regulates DNA double-strand break-induced checkpoint activation and repair. Mol Cell Biol. 2013 May;33(9):1830-44. doi:10.1128/MCB.01521-12. Epub 2013 Feb 25. PubMed PMID: 23438602; PubMed Central PMCID: PMC3624173. (http://www.ncbi.nlm.nih.gov/pubmed/?term=23438602)

  12. Ghodke I, Muniyappa K. Processing of DNA double-stranded breaks and intermediates of recombination and repair by Saccharomyces cerevisiae Mre11 and its stimulation by Rad50, Xrs2, and Sae2 proteins. J Biol Chem. 2013 Apr 19;288(16):11273-86. doi: 10.1074/jbc.M112.439315. Epub 2013 Feb 26. PubMed PMID:23443654; PubMed Central PMCID: PMC3630844.(http://www.ncbi.nlm.nih.gov/pubmed/?term=23443654)

  13. Gangadhara Reddy S, Divya K, Bharadwaja Kirti P, Prakash Babu P (2013) Novel Antiproliferative and Antioxidant Role of BjAnn1, a Mustard Annexin Protein in Human Glioblastoma Cell Lines. J Cancer Sci Ther 5:256-263. doi: (http://omicsonline.org/1948-5956/JCST-05-256.php?aid=15357)

  14. Samaddar S, Dutta A, Sinharoy S, Paul A, Bhattacharya A, Saha S, Chien KY,Goshe MB, DasGupta M. Autophosphorylation of gatekeeper tyrosine by symbiosis receptor kinase. FEBS Lett. 2013 Sep 17;587(18):2972-9. doi:10.1016/j.febslet.2013.07.050. Epub 2013 Aug 17. PubMed PMID: 23962520. (http://www.ncbi.nlm.nih.gov/pubmed/23962520)