This assay employs an antibody specific for Anti-human HIF1α coated on a 96-well plate. Standards and samples are pipetted into the wells and HIF1α present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human HIF1α antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HIF1α bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This immunoassay kit allows for the in vitro quantitative determination of Human HIF1α concentrations in serum, plasma and other biological fluids.
Detection Range: 0.156 – 10 ng/ml
Sensitivity: < 0.094 ng/ml
