This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IκBα antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IκBα detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IκBα amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450nm in a microplate reader. Concentration of Human IκBα can be calculated using the standard curve.
This immunoassay kit allows for the in vitro quantitative determination of Human IκBα concentrations in serum, plasma and other biological fluids.
Detection Range: 0.156 – 10 ng/ml
Sensitivity: < 0.094 ng/ml
