Quick-blots (Mouse Tissues)

Product code: 16-1001

Application : WB
Reactivity : Mouse

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Size
Price
1 Membrane
7,000.00 


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Shipping Info:

Order now and get it on next 3-4 working days* (Major Cities)- Free shipping

Amount : 1 Membrane

Description:  Quick-blotsTM is Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 μg total protein per lane). Quick-blotsTM is a very simple method to identify different mouse proteins. 

 
Content : 1 pre-transferred membrane
Storage condition : Store unopened at RT. For long-term, store at 4⁰C.

Quick-blotsTM Protocol:

  1. Activate the membrane with Methanol for 1-2 min and then wash the blots with TBST (25 mM Tris-Cl, pH 8.0; 125 mM NaCl; 0.1% Tween 20)  buffer thrice to get rid of extra methanol.
  2. Block the membrane for 1 hour in 5% skimmed milk ( in TBST) to avoid non specific binding of antibodies.
  3. Wash the membranes 2 times in TBST and incubate with primary antibodies prepared in 1% skimmed milk for 2 hr in RT/ over night at 4⁰C.
  4. After primary incubation, wash the membranes for 1 hour in TBST at RT. (Buffer should be changed in 30 min interval)
  5. Incubate the membranes in appopriate Secondry antibody for 1 hour.
  6. After secondary incubation, wash the membranes for 2 hours (Buffer should be changed in 30 min interval). Then develop the blots with Chemiluminescent Substrate and interpret the results in photographic film .


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Quick-blots (Mouse Tissues)

Product code: 16-1001
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