Quick-blots (Rat Tissues)

Product code: 16-1002

Application : WB
Reactivity : Rat

Shipping Info:

2000 (throughout India)
Order now and get it on next 3-4 working days* (Major Cities)

Size
Price
1 Membrane
7,000.00 


Bulk Order


Shipping Info:

2000 (throughout India)

Order now and get it on next 3-4 working days* (Major Cities)


Amount : 1 Membrane

Quick-blotsTM is Ready-to-use PVDF membrane with 11 Rat tissue lysates (20 µg total protein per lane). Quick-blotsTM is a very simple method to identify different proteins from rat tissues. 

Content : 1 pre-transferred membrane
Storage condition : Store unopened at RT. For long-term, store at 4⁰C.

Protocol:

1. Activate the membrane with Methanol for 1-2 min and then wash the blots with TBST (25 mM Tris-Cl, pH 8.0; 125 mM NaCl; 0.1% Tween 20) buffer thrice to get rid of   extra methanol.

 2. Block the membrane for 1 hour in 5% skimmed milk ( in TBST) to avoid non specific binding of antibodies.

3. Wash the membranes 2 times in TBST and incubate with primary antibodies prepared in 1% skimmed milk for 2 hr in RT/ over night at 4oC.

4. After primary incubation, wash the membranes for 1 hour in TBST at RT. (Buffer should be changed in 30 min interval)

5. Incubate the membranes in appopriate Secondry antibody for 1 hour.

6. After secondary incubation, wash the membranes for 2 hours (Buffer should be changed in 30 min interval). Then develop the blots with Chemiluminescent Substrate and    interpret the results in photographic film.


Related Products

Quick-blots (Rat Tissues)

Product code: 16-1002
mg

Can't read the image? click here to refresh.