Breast: cancer-normal

Product code: 16-2009

Application : IHC

Shipping Info:

2000 throughout India. Order now and get it on next 3-4 weeks* (Major Cities).

Size
Price
1 Slide
11,800.00 


Bulk Order


Shipping Info:

2000 throughout India. Order now and get it on next 3-4 weeks* (Major Cities).

Amount : 1 Slide
Core diameter           :   2.0 mm
Section thickness              :   4 micrometer
Slide orientation  :   In most of the slides with 59 or 60 cores, the orientation is as below unless indicated otherwise. # 60 location is usually filled with carbon for orientation. 

Storage condition : Individual slide is put in an air-tight pack with inert gas. If the slides are stored at 4 degree, they are good for up to one year.

How processed

  •  Tissues were initially fixed with formalin except for some of the animal tissues.
  •  Then, dehydrated with gradient ethanol; typically 1 hour each progressive steps; 70%, 90%,  95%, 99%, 100% x 3 times.
  •  Cleared by xylene, three changes for 1 hour each.
  •  Infiltrated with 60°C paraffin, three changes for 1 hour each
  •  Sectioned by microtome in 4 micrometer  thickness and put on Superfrost plus slides.

Before use

  • Dry slides for 1 hour in a oven at 60 degree.
  • Dewax slides in xylene for 4 minutes x 5 times.
  • Hydrate slides in 100%, 95% and 75% ethanol for 3 minutes x 2 times each.
  • Immerse slides in tap water for 5 minutes.

Applications

  • Immunohistochemistry
  • Fluorescent in situ hybridization (FISH)
  • mRNA in situ hybridization
  • miRNA in situ hybridization
  • TUNEL for apoptosis
  • Nucleic acid extraction


Breast: cancer-normal

Product code: 16-2009
mg

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