|
|
| Gene : |
MUC1 |
| Gene ID : |
4582 |
| Uniprot ID : |
P15941 |
| Alternative Name : |
MUC1, PUM |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
GP1.4 + E29 |
| Isotype : |
Mouse IgG1, kappa + Mouse IgG2a, lambda |
| Immunogen Information : |
Human milk fat globule membranes (GP1.4); Delipidated extract of human milk fat globule membranes (E29) |
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. The α subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The β subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
