Monoclonal Antibody to Neurofilament (NF-L) (Neuronal Marker)(Clone : NR-4)

Product code: 36-1512

Clonality : Monoclonal
Application : IF, FACS, IHC
Reactivity : Human, Rat

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg


Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : NEFL
Gene ID : 4747
Uniprot ID : P07196
Alternative Name : NEFL, NF68, NFL
Format : Purified
Amount : 100 µg
Clone name : NR-4
Isotype : Mouse IgG1, kappa
Immunogen Information : Crude neurofilament prepaRation from porcine spinal cord
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Immunofluorescence (1-2ug/ml); Flow Cytometry (1-2ug/million cells); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);


Monoclonal Antibody to Neurofilament (NF-L) (Neuronal Marker)(Clone : NR-4)

Product code: 36-1512
mg

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