Monoclonal Antibody to Dnmt1 (Clone: ABM13B2)

Product code: 10-7002

Clonality : Monoclonal
Application : IHC, FACS, WB
Reactivity : Mouse, Human

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Gene : DNMT1
Gene ID : 1786
Uniprot ID : P26358
Alternative Name : DNMT1, AIM, CXXC9, DNMT
Format : Purified
Amount : 100 µg
Clone name : ABM13B2
Isotype : Mouse IgG1 Kappa
Immunogen Information : A partial length recombinant DNMT1 protein (amino acids 128-429) was used as the immunogen for this antibody.

Dnmt1(DNA (cytosine-5-)-methyltransferase 1 ) is one of the most abundant DNA methyltransferase in mammalian tissues, where it associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. Dnmt1 comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2). The C-terminal domains of mammalian Dnmts contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. Dnmt1 maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity. The molecules interacting with Dnmt1, including RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules are involved in chromatin organization, DNA repair, cell cycle regulation, and apoptosis.

Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, FACS analysis: 0.5 µg/10^6 cells


Monoclonal Antibody to Dnmt1 (Clone: ABM13B2)

Product code: 10-7002
mg

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