|
|
| Gene : |
AFP |
| Gene ID : |
174 |
| Uniprot ID : |
P02771 |
| Alternative Name : |
AFP, HPAFP |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM334 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Alpha fetoprotein (AFP) purified from serum of a hepatoma patient |
It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes);
