Monoclonal Antibody to CD59 / Complement Regulatory Protein / Protectin(Clone : SPM616)

Product code: 36-1954

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human

Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


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Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : CD59
Gene ID : 966
Uniprot ID : P13987
Alternative Name : CD59, MIC11, MIN1, MIN2, MIN3, MSK21
Format : Purified
Amount : 100 µg
Clone name : SPM616
Isotype : Mouse IgG1 Kappa
Immunogen Information : Recombinant full-length human CD59 protein
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Functional Studies (Order Ab without Azide); Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1µg/ml); Immunohistology (Formalin-fixed) (1-2µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to CD59 / Complement Regulatory Protein / Protectin(Clone : SPM616)

Product code: 36-1954
mg

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