|
|
| Gene : |
KRT6A |
| Gene ID : |
3853 |
| Uniprot ID : |
P02538 |
| Alternative Name : |
KRT6A, K6A, KRT6D |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
KRT/457 |
| Isotype : |
Mouse IgG1 |
| Immunogen Information : |
KeRatin-enriched prepaRation from cultured human epithelial cells |
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (0.5-1.0µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistology (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
