|
|
| Gene : |
EGFR |
| Gene ID : |
1956 |
| Uniprot ID : |
P00533 |
| Alternative Name : |
EGFR, ERBB, ERBB1, HER1 |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
31G7 + GFR1195 |
| Isotype : |
Mouse IgG2a, kappa |
| Immunogen Information : |
Human EGFR purified from A431 cells (31G7); Recombinant human EGFR protein (GFR1195) |
This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, βcellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Digest formalin-fixed tissues with Protease (Sigma Cat. #P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)
