|
|
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
Hyb-8 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Biotinylated sheep immunoglobulin |
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Confocal Microscopy; In situ Nucleic Acid Hybridization; Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
