Monoclonal Antibody to Calponin-1 (Smooth Muscle Marker)(Clone : CNN1/832 + CALP)

Product code: 36-1064

Clonality : Monoclonal
Application : FACS, IF, IHC
Reactivity : Human, Rat

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2000 throughout India Order now and get it on next 3-4 weeks

Size
Price
100 µg
15,000.00 


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Shipping Info:

2000 throughout India Order now and get it on next 3-4 weeks

Gene : CNN1
Gene ID : 1264
Uniprot ID : P51911
Alternative Name : CNN1
Format : Purified
Amount : 100 µg
Clone name : CNN1/832 + CALP
Isotype : Mouse IgG
Immunogen Information : Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Immunohistology (Formalin-fixed) (0.5-1µg/ml for 30 minutes at RT); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.


Monoclonal Antibody to Calponin-1 (Smooth Muscle Marker)(Clone : CNN1/832 + CALP)

Product code: 36-1064
mg

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