Mouse Cystathionine Gamma-Lyase (CTH) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Mouse Cystathionine gamma-lyase concentrations in serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Target Cystathionine Gamma-Lyase (CTH)
Reactivity Mouse
Tested Applications ELISA
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Validity The validity for this kit is 6 months.
Stability The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate
UniProt Primary AC Q8VCN5 (UniProt, ExPASy)
UniProt Secondary AC Q6H324
UniProt Entry Name CGL_MOUSE
Gene Symbol CTH
KEGG mmu:107869
String 10090.ENSMUSP00000113672
Test Range 0.78 ng/ml - 50 ng/ml
Sensitivity < 0.4 ng/ml
Standard Form Lyophilized
Detection Method Colorimetric
Assay Type Sandwich
Assay Data Quantitative
Sample Type Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Assay Principle This kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient CTH will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow colour is proportional to the CTH amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of CTH can be calculated.
