|
|
Alternative Name : |
Sulfate adenylyltransferase, EC 2.7.7.4, Sulfate adenylate transferase, SAT, ATP-sulfurylase, Methionine-requiring protein 3, ATPS. |
Amount : |
50 IU |
Source : Escherichia Coli. Adenosine 5' Triphosphate Sulfurylase Yeast Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Mw of 57.7 kDa. Adenosine 5' Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5'-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5 Triphosphate Sulfurylase is purified by proprietary chromatographic techniques. ATP sulphurylase synthesizes adenosine 5'-sul-phatophosphate from ATP and inorganic SO42-. This is the first reaction of a two step sequence in the formation of 'active sulphate', adenosine 3'-phosphate5'-sulphatophosphate, which is a sulphate donor for a wide variety of compounds and is also involved in the reduction of sulphate.
Spin vial before opening. Reconstitute ATP sulphurylase with 5mM NaP pH-7.5 & 0.75mM DTT at a concentration ranging from 0.1mg – 1mg per ml. Can be diluted further into other aqueous buffers. pH range between 7.0 – 8.5 is best. 12 Units/mg.