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Alternative Name : |
Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142. |
Amount : |
50 µg |
Source : Chinese Hamster Ovary Cells(CHO).
Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques.
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg.