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| Alternative Name : |
EC 1.8.1.7,Glutathione reductase mitochondrial,GR,GSR,MGC78522 |
| Amount : |
200 Tests |
Detection method : Colorimetric
Sample type : Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate
Assay type : Enzyme activity
Sensitivity : > 0.1 mU/ml
Range : 0.1 mU/ml - 40 mU/ml
Assay time : 0h 40m
Glutathione Reductase Assay Kit (abg83461) is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5'-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1-40 mU/ml GR in various samples.
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress.
