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| Alternative Name : |
IGFBP-5, IBP-5, IGF-binding protein 5. |
| Amount : |
25 µg |
Source : Escherichia Coli.
IGFBP5 Human Recombinant produced in E.Coli is a single, non-glycosylated, homodimeric polypeptide chain containing 252 amino acids and having a molecular mass of 28.6 kDa. IGFBP5 is purified by proprietary chromatographic techniques.
IGFBP5 is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
It is recommended to reconstitute the lyophilized Insulin-Like Growth Factor Binding Protein-5 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50, calculated by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is < 0.4µg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15ng/ml of rHuIGF-II.
