|
|
| Gene : |
B2M |
| Gene ID : |
567 |
| Uniprot ID : |
P61769 |
| Alternative Name : |
B2M, CDABP0092, HDCMA22P |
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
B2M/961 |
| Isotype : |
Mouse IgG2b, kappa |
| Immunogen Information : |
Full length recombinant human B2M protein |
Recognizes a protein of 12kDa, identified as β-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an α heavy chain that contains three subdomains (α1, α2, α3) and a non-covalent associating light chain, known as β-2-Microglobulin. β-2-Microglobulin associates with the α3 subdomain of the α heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The α1 and α2 domains of the α heavy chain form the peptide antigen-binding cleft. Mutations in the β-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.
Flow Cytometry (0.5-1ug/million cells); ,Immunofluorescence (1-4ug/ml); Western Blot (0.5-2ug/ml);,Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),
