|
|
| Format : |
Purified |
| Amount : |
100 µg |
| Clone name : |
SPM375 |
| Isotype : |
Mouse IgG1, kappa |
| Immunogen Information : |
Biotinylated sheep immunoglobulin |
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Confocal Microscopy; Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); In situ Nucleic Acid Hybridization; Western Blot; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.)
